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It is important to have well prepared, competent and knowledgeable pharmacists as they help tackle the current public health problem of antimicrobial resistance (AMR). The purpose of this work was to test how much fifth year pharmacy students in Northern Nigeria know about antibiotics, resistance to microorganisms and the proper use of antibiotics.We conducted a paper-based, descriptive cross-sectional study that included questions asked from July to September 2021. Each person filled out the questionnaire independently and it had four sections. In the first section, I asked about respondents’ personal information and section B centered on their awareness of antibiotics and AMR. Part C had six questions that measured people’s knowledge of various AMS areas and the final part tested their ability to use antibiotics and how informed they are about these subjects. These findings were described using statistical methods.There were 164 questionnaires collected as part of the results. The majority of those interviewed were men and teenagers between 21 and 25. While some understanding about antibiotics and AMR was present, a number of mistakes about them were found as well. Even though only 80 students (48.8%) knew what AMS is, a high percentage of them understood AMS’s aims and the roles on the AMS team. Most respondents admitted that their knowledge of antibiotics, AMR or AMS was not enough for their future work and more than 90% of them wanted to learn more about these issues.In sum, while much of the participants understood these concepts, there were still a number of areas where they lacked understanding. Undergraduate pharmacy education should be improved in these areas.